Using a DR resistant tumor cell line.

Using a DR – resistant tumor cell line, in which the PTEN gene could be on or off, Kalaany checks whether a change in the PTEN gene alone may affect the sensitivity of a tumor to DR. When the PTEN switched off, the switched off, the cancer cells were not unaffected by dietary restriction, and the tumor size also increases the control and DR mice. But when PTEN was switched, so that the restoration of normal function to the PI3K pathway, the cells were sensitized dietary restriction and tumor size was smaller in the DR group..

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